In the ever-evolving landscape of scientific research, the demand for ethical and sustainable solutions has grown exponentially. As researchers, it is our responsibility to prioritize the welfare of animals and minimize our impact on the environment. This is why we are proud to introduce a comprehensive range of animal component-free cell culture reagents from our supplier partners, carefully designed to meet your research needs while upholding ethical standards.
Using animal-free cell culture reagents offers distinct advantages. Not only do these products eliminate the need for animal-derived components, but they also mitigate the risk of potential contamination. This ensures cleaner, more reliable results for your experiments. By making the switch to animal-free reagents, you can enhance the reproducibility and robustness of your research outcomes, contributing to the advancement of scientific knowledge on a stable foundation.
At Krishgen, we understand the significant impact that the choices we make today have on the future of scientific research. That’s why we have committed ourselves to partnering with companies from around the world manufacturing high-quality, animal-free alternatives that provide optimal performance without compromising on ethics or sustainability. We firmly believe that innovative research is not mutually exclusive from humane practices. By embracing animal-free cell culture reagents, you can be part of a growing movement dedicated to conducting research that respects both animal welfare and the environment.
Krishgen’s partners offering Animal-Free Media, Supplements, and Growth Factors. Browse the tables below to learn more and click through to our partners’ website for more details or get in touch with our technical team for support choosing the right products for you.
Cell Culture Media from Sino Biologicals, USA
1) HEK293 Media
| Product Name | Type | Product Description |
|---|---|---|
| M293CD1 | SMM 293-CD1 Expression Medium | Latest generation product for HEK293 cell suspension culture, transient protein expression, and lentiviral packaging |
| M293TII | SMM 293-TII Expression Medium | New-generation product for HEK293 cell suspension culture, transient protein expression, and lentiviral packaging |
| M293TI | SMM 293-TI Expression Medium | Primary product for HEK293 cell suspension culture, transient protein expression, and lentiviral packaging |
| M293-SUPI | SMS 293-SUPI supplement | Used in combination with culture medium (Cat#: M293CD1, M293TII, M293TI) and added after transfection to increase expression yields |
2) Insect Cell Culture Media
| Product Name | Type | Product Description |
|---|---|---|
| MSF-AF | SIM SF Animal-Component-Free Medium | Sf9/Sf21 cell culture, baculovirus packaging, and protein expression |
| MHF-AF | SIM HF Animal-Component-Free Medium | Hi-5 cell culture and protein expression |
| MCHOSI | SMM CHO-SI Expression Medium | CHO-K1 cell screening, culture, and stable protein expression in DHFR systems |
| MCHO-SUPI | SMS CHO-S-SUPI supplement | Used in combination with culture medium (Cat#: MCHOSI) to increase expression yields |
3) CHO Cell Culture Media
| Product Name | Type | Product Description |
|---|---|---|
| MCHOGSI | SMM CHO-GSI Expression Medium | CHO-K1 cell screening, culture, and stable protein expression in GS systems |
| MCHOGS-C3 | SMM CHOGS-C3 Expression Medium | CHO-K1 cell screening, culture, and stable protein expression in GS systems |
| MCHO-GS-SUPI | SMS CHO-GS-SUPI supplement | Used in combination with medium (Cat#: MCHOGSI, MCHOGS-C3) to increase expression yields |
4) Hybridoma Cell Culture Media
| Product Name | Type | Product Description |
|---|---|---|
| MCHOTII | SMM CHO-TII Expression Medium | CHO-S cell culture and transient protein expression |
| MCHOT3 | SMM CHOT3 Expression Medium | CHO-S cell culture and transient protein expression |
| MCHO-SUPA | SMS CHO-SUPA supplement | Used in combination with culture medium (Cat#: MCHOTII, MCHOT3) to increase expression yields |
5) Vero Cell Culture Media
| Product Name | Product Description |
|---|---|
| OptiVERO® | OptiVERO® was specifically optimized for VERO-based virus production in both 2D and 3D culture formats. This media formulation is supplied as a concentrated frozen protein supplement with a base media. OptiVERO does not contain any blood-derived components and does not contain plant hydrolysates and is blood-free and chemically defined. |
Other Animal Free Cell Media from Sciencell
| Product Name | Product Description |
|---|---|
| Fibroblast Medium | Fibroblast Medium-animal component free (FM-acf), when used with Fibroblast Growth Supplement-animal component free (FGS-acf, Cat #2372), is a complete medium designed for optimal growth of normal fibroblasts in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors and trace minerals. The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively supports the growth of normal fibroblasts in vitro. |
| Keratinocyte Medium | Keratinocyte Medium-animal component free (KM-acf), when used with Keratinocyte Growth Supplement-animal component free (KGS-acf, Cat #2172), is a complete medium designed for optimal growth of normal keratinocytes in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors and trace minerals. The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively supports the growth of normal keratinocytes in vitro. |
| HematoGro Medium | HematoGro Medium'Animal Component Free (HemGM-ACF), when used with HematoGro Supplement'Animal Component Free (HemGS-ACF, Cat #5562) is a complete medium designed for short-term maintenance of hematopoietic cells in vitro, including bone marrow mononuclear and peripheral blood cells. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, hormones, organic and inorganic compounds and recombinant human serum albumin. The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. This medium does not contain serum, growth factors, cytokines or animal-derived components. Supplementation with additional factors may be used to support the proliferation or differentiation of select cell subpopulations. |
Mesenchymal and Pluripotent Stem Cell Media from PanBiotech and Sciencell, USA
| Product Name | Product Description |
|---|---|
| PowerStem MSC1, Serum-free medium for human mesenchymal stem cells (huMSC) | PowerStem MSC1 is an easy to use xeno-free medium without animal derived components (ADCF) for cultivation and proliferation of human mesenchymal stem cells (hMSC). PowerStem MSC1 is especially designed for the proliferation of human mesenchymal stem cells without differentiation. PowerStem MSC1 supports longterm growth of MSC and preserves their multi-lineage potential. In addition, MSC cultured in PowerStem MSC1 expands faster and shows a significant reduction in hematopoietic cell contamination at early passages compared to serum-based media. To differentiate the proliferated MSC into different cells types the relevant protocols and differentiation factors should be used. Both the PowerStem MSC1 basal medium and two PowerStem MSC1 growth supplements are guaranteed stable for 12 months when properly stored. PowerStem MSC1 complete medium (basal + supplements) is stable for 1 month when stored in the dark at 2-8° C. We do not recommend using the complete medium beyond one month. Do not freeze complete PowerStem MSC1 medium. |
| Panexin CD, Serum Replacement with Defined Components | Panexin CD is an innovative brand-new product, developed based on the current state of science and 15 years of experience in developing serum replacements. Compared to Pan Biotech’s other serum replacements, Panexin CD contains only chemically defined components.  |
| Mesenchymal Stem Cell Medium | Mesenchymal Stem Cell Medium-animal component free (MSCM-acf), when used with Mesenchymal Stem Cell Growth Supplement-animal component free (MSCGS-acf, Cat #7572), is a chemically defined medium designed for optimal growth of normal mesenchymal stem cells in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors and trace minerals. The medium is xeno-free and does not contain any animal-or human-origin materials. The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively supports the growth of normal mesenchymal stem cells in vitro. |
| STEMium® Human Pluripotent Stem Cell Growth Medium | STEMium-acf™ is a animal component free medium designed for optimal growth of human embryonic stem cells and induced pluripotent stem cells under feeder-free conditions. It is a sterile, liquid medium containing essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors and trace minerals. It is made with synthetic and plant-derived components and is free of bovine and other animal-derived components. The medium is bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/ 95% air. STEMium™ is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively promotes growth of normal human pluripotent stem cells in vitro. This medium should be used in conjunction with Geltrex™ or BD Matrigel™ for feeder-free culture conditions. |
| Human Pluripotent Stem Cell Growth Supplement 50X | StemGS-acf® 50X human pluripotent stem cell growth supplement is a animal component free medium supplement designed for the optimal growth of human pluripotent stem cells in vitro. It is a sterile, concentrated (50X) solution which contains recombinant growth factors, hormones, and proteins. It is made with synthetic, plant-derived components and is free of bovine and other animal-derived components. The supplement is formulated (quantitatively and qualitatively) to provide an optimally balanced growth environment that maximally promotes the growth of human pluripotent stem cells in vitro. Use Geltrex™ or BD Matrigel™ for feeder-free culture conditions. StemGS-acf® 50X is designed as an additive for STEMium-acf™ basal medium (Cat. No. 5821) and should be used in conjunction with that medium. |
FBS Replacements with PL Biosciences
Each batch of ELAREM™ Human Platelet Lysates is produced from large pools of platelet units to ensure batch-to-batch consistency and enable reproducible conditions. It manufactured from platelet units obtained from healthy blood donors at licensed blood centers in the US / EU.
| Product Name | Type | Product Description |
|---|---|---|
| ELAREM™ Perform I Research Grade | Human Platelet Lysate, anticoagulant required – EU origin | ELAREM™ Perform is a human Platelet Lysate of EU origin supporting in vitro expansion of various primary cells and cell lines. The xeno-free cell culture supplement meets the requirements of academic and industrial cell expansion: The safe and performance-increasing cell growth promoter ensures efficient lab processes and a xeno-free cell environment. As a result, ELAREM™ Perform assures an easy transition of research results into clinical applications. |
| ELAREM™ Perform-FD I GMP Grade | Fibrinogen-depleted Human Platelet Lysate, no anticoagulant required – GMP Grade – US origin | ELAREM™ Perform-FD GMP Grade is a fibrinogen-depleted human Platelet Lysate of US origin. As a cell culture supplement, ELAREM™ Perform-FD GMP Grade supports in vitro expansion of various primary cells and cell lines. Due to the fibrinogen-depletion process, ELAREM™ Perform-FD GMP Grade does not require anticoagulant addition. As a result, application workflows are simplified. Traces of a xeno-free anticoagulant may be present in the human Platelet Lysate. ELAREM™ Perform-FD GMP Grade is manufactured under GMP conform conditions using high quality raw materials. Furthermore, the production process is performed within a closed system using sterile connections. |
| ELAREM™ Perform-FD I Research Grade | Fibrinogen-depleted Human Platelet Lysate, no anticoagulant required – US origin | ELAREM™ Perform-FD is a fibrinogen-depleted human Platelet Lysate of US origin. As a cell culture supplement, ELAREM™ Perform-FD supports in vitro expansion of various primary cells and cell lines. Due to the fibrinogen-depletion process, ELAREM™ Perform-FD does not require anticoagulant addition. As a result, application workflows are simplified. Traces of a xeno-free anticoagulant may be present in the human Platelet Lysate. |
| ELAREM™ Perform-FD PLUS I Research Grade | Fibrinogen-depleted, clot-free Human Platelet Lysate, no anticoagulant required or added – EU origin | ELAREM™ Perform-FD PLUS Research Grade is a fibrinogen-depleted and anticoagulant-free Human Platelet Lysate of EU origin. As a xeno-free and easy-to-use cell culture supplement, ELAREM™ Perform-FD PLUS supports the efficient and safe in vitro expansion of various primary cells and cell lines. Due to a unique fibrinogen-depletion technique, ELAREM™ Perform-FD PLUS is a clot-free and anticoagulant-free Human Platelet Lysate. The product neither requires the addition of anticoagulants in cell culture nor during manufacturing. That makes ELAREM™ Perform-FD PLUS a ready-to-use Human Platelet Lysate that is widely applicable, since the absence of anticoagulants offers even more convenient cell growth conditions. |
| ELAREM™ Matrix Kit | Human Platelet Lysate-based gel scaffold – 2D/3D cell culture system – EU origin | ELAREM™ Matrix Kit provides a gel scaffold based on human Platelet Lysate that is suitable for 2D and 3D cell culture applications. The kit consists of ELAREM™ Matrix, a human Platelet Lysate gel, and ELAREM™ Accelerator, a xeno-free additive inducing the instant gelation of the scaffold. This combination enables the three-dimensional embedding of cells within the gel scaffold as well as the two-dimensional cell seeding on a viscous surface. The patent-protected technology provides both a nutrient source and a viscous scaffold in one – resembling the natural cell environment. Cells benefit a uniform distribution of growth factors. Furthermore, cells grow homogeneously in several layers, which minimizes contact inhibition with artificial biomaterials. Consequently, the cell morphology is closer to its native morphology. The viscous consistency of the cell culture matrix enables non-enzymatic cell passaging with a convenient harvesting and reseeding procedure. |
Insulin / Transferrin Cell Culture Media Supplements from Invtria, USA
Optimize cell-culture performance of chemically defined media.
| Product Name | Product Description |
|---|---|
| ITSE Animal-Free | ITSE Animal-Free® is a recombinant insulin and transferrin cell culture 100x media supplement. ITSE AF contains four components that are typically required for optimal cell growth in serum-free or reduced serum media. Insulin has cell signaling functions and promotes the uptake of glucose and amino acids. Transferrin is a universal source of iron for cells that is non-toxic carrier of iron and reduces the generation of toxic free-radicals and peroxide. Selenium is required for the activity of glutathione peroxidase, thioredoxin reductase, and other antioxidant enzymes. Ethanolamine is a phospholipid precursor that improves the performance of cells in serum-free media and is required for the growth of some cell types. |
| ITS AF | ITS Animal-Free™ is a recombinant insulin and transferrin 100x cell culture media supplement that is ideal for serum-free or reduced serum cell culture applications. Similar to ITSE, ITS AF contains three components that are typically required for optimal cell growth in serum-free or reduced serum media but lacks ethanolamine. |
| ITSE + A | ITSE + Albumin Animal-Free™ is a recombinant blood-free insulin transferrin albumin 100x cell culture media supplement that also contains selenium and ethanolamine. ITSE + Albumin is intended to reduce or replace FBS or human serum in cell culture media. |
Animal Free Proteins and Enzymes
All of the protein components are manufactured biosynthetically using recombinant DNA technology in an animal-free host. These products enable the reduction of serum and serum-derived contaminants from cell culture media and support improved ex vivo cellular analyses. InVitria’s Recombinant insulin-transferrin supplements are recommended for use with most cultured cell types, including:
- Hybridoma cells
- CHO cells
- Stem cells
- VERO
- HEK293
- Fibroblasts
- Primary cell isolation and passage
- Immortalized cell lines
| Product Name | Product Description |
|---|---|
| Lacromin® – Recombinant human holo-lactoferrin | Lacromin® is a cell culture media supplement that increases cell growth while protecting cells from apoptosis (programmed cell death) in select cell types via activation of specific cellular signaling similar to that of Insulin-Like Growth Factor (IGF). Lacromin is particularly well-suited as a cell culture media supplement for CHO and HEK cells and select primary and pluripotent cell types. |
| Leukemia Inhibitory Factor | Leukemia Inhibitory Factor is commonly used to replace feeder cells in mouse embryonic stem cell culture. This rhLIF product and has the lowest endotoxin and highest purity of the commercial LIF products available today. |
| Optiferrin® – Recombinant human transferrin | Optiferrin® is recombinant human transferrin that is completely blood-free. Optiferrin’s performance is equivalent to human holo transferrin in cell culture and offers significant regulatory advantages due to its safety and consistency. Optiferrin is suitable for a broad range of cell types that include stem, primary, and bioproduction cells. |
| Lysobac® – Recombinant human lysozyme | Lysobac® is a recombinant human lysozyme that is suitable for uses as a preservative in foods and beverages, diagnostic applications, bioprocessing, and life science research. Lysobac is four times more active per mg for lysing Micrococcus and E. coli, when compared with the highly variable chicken lysozyme and thus is a more effective as a preservative. Lysobac also eliminates any concerns regarding consumers with egg allergies. |
| Optibumin® – Recombinant human albumin | Optibumin® is the highest-purity rHSA available on the market. With exceptionally low levels of albumin aggregates and lipids, and an exceptionally high concentration of free-thiol/Cys-34 mercapto-albumin, Optibumin is optimized for the most demanding of applications, including solubilization or bioconjugation of therapeutic entities in order to improve their pharmacokinetics, inclusion in medical devices, support of in vitro fertilization and cord blood cryopreservation, as well as iPSC and hESC. For applications requiring safety and uncompromising quality with a USA-origin, it is the product of choice. |
| Cellastim S® – Recombinant human albumin | Cellastim S® is recombinant human serum albumin that is completely blood-free and has been optimized specifically to enhance the performance in animal-free cell culture media due to the favorable lipid profile and other engineered attributes of the molecule. Cellastim S is ideally suited for a variety of different stem, primary, and production cell types that include immune cells, VERO, Mesenchymal Stem Cells, HEK, and CHO. Each lot tested in cell culture systems to ensure consistent quality and performance. |
| Exbumin® – Recombinant human albumin excipient | Exbumin® is a recombinant human serum albumin that is manufactured using good manufacturing practices at an ISO9001:2015 certified facility in Kansas, USA. It is blood-free and has been used in hundreds of thousands of injections. It is one of the few excipients available to improve viral stability for vaccines and gene therapies. Exbumin is also used to maintain tissue viability during cryo-preservation or storage. And, Exbumin helps to stabilize cells and tissue used in cell therapy and regenerative medicine applications. Exbumin has been approved for use as an excipient in injectable therapeutics by regulatory agencies. |
Benefits of Animal-Free Cell Culture Reagents
When it comes to cell culture research, the quality and consistency of your results are paramount. Animal-free cell culture reagents play a crucial role in ensuring reliable outcomes and providing several key advantages.
By eliminating animal-derived components from your cell culture protocols, you can significantly reduce the risk of contamination. Animal-free reagents offer a clean and controlled environment, minimizing the impact of external variables on your research. This translates to enhanced reproducibility, allowing you to confidently confirm and build upon previous findings.
Moreover, animal-free cell culture reagents help overcome the ethical concerns associated with traditional methods. As a responsible researcher, you can align your work with ethical guidelines and contribute to the reduction of animal use in scientific experiments. This not only demonstrates your commitment to compassionate research but also helps shape a culture of ethical scientific practice.
In addition to ethical considerations, animal-free reagents offer improved consistency and reliability. Each batch is meticulously formulated to ensure optimal performance, resulting in more accurate and meaningful data. Whether you are working with primary cells, cell lines, or stem cells, animal-free cell culture reagents provide a stable foundation for your research, enabling you to unlock the full potential of your experiments.
Make the switch to animal-free cell culture reagents today and experience the transformative benefits they offer. Elevate your research potential while upholding your commitment to ethical practices and environmental sustainability.